Assembly
ASM2016v1
BioProject PRJNA28857 · BioSample SAMN02603538
GENOME-RESOLVED EVIDENCE
GCA_000020165.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3847969 bp · GC 27.500%
Completeness 98.940% · contamination —%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 1/3 | 0.333 | Partial/absent |
| ethanol_entry_support | 0/1 | 0.000 | Partial/absent |
| fatty_acid_biosynthesis_core | 2/8 | 0.250 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| accA | acetyl-CoA carboxylase carboxyltransferase alpha | 1 |
| accD | acetyl-CoA carboxylase carboxyltransferase beta | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 2 |
| bcd | butyryl-CoA dehydrogenase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| ldhCLL_A2569 | ACD24272.1 | OG0000131 | CP001056.1:2678443–2679393 - | L-lactate dehydrogenase [Clostridium botulinum B str. Eklund 17B (NRP)] |
| ldhCLL_A3511 | ACD23473.1 | OG0000131 | CP001056.1:3717614–3718561 - | L-lactate dehydrogenase [Clostridium botulinum B str. Eklund 17B (NRP)] |
| hbdCLL_A0377 | ACD23317.1 | OG0000229 | CP001056.1:385061–385909 + | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum B str. Eklund 17B (NRP)] |
| thlCLL_A0490 | ACD24544.1 | OG0000234 | CP001056.1:490638–491819 + | acetyl-CoA acetyltransferase [Clostridium botulinum B str. Eklund 17B (NRP)] |
| accDCLL_A1155 | ACD23491.1 | OG0000403 | CP001056.1:1182727–1183602 + | acetyl-CoA carboxylase, carboxyl transferase, beta subunit [Clostridium botulinum B str. Eklund 17B (NRP)] |
| accACLL_A1156 | ACD23776.1 | OG0000885 | CP001056.1:1183595–1184419 + | acetyl-CoA carboxylase, carboxyl transferase, alpha subunit [Clostridium botulinum B str. Eklund 17B (NRP)] |
| lctPCLL_A1363 | ACD22331.1 | OG0001228 | CP001056.1:1397041–1398585 + | L-lactate permease [Clostridium botulinum B str. Eklund 17B (NRP)] |
| bcdCLL_A0374 | ACD23288.1 | OG0001311 | CP001056.1:381987–383126 + | butyryl-CoA dehydrogenase [Clostridium botulinum B str. Eklund 17B (NRP)] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.