Assembly
ASM2028v1
BioProject PRJNA28855 · BioSample SAMN02603537
GENOME-RESOLVED EVIDENCE
GCA_000020285.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3659644 bp · GC 27.500%
Completeness 98.860% · contamination —%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 1/3 | 0.333 | Partial/absent |
| ethanol_entry_support | 0/1 | 0.000 | Partial/absent |
| fatty_acid_biosynthesis_core | 2/8 | 0.250 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| accA | acetyl-CoA carboxylase carboxyltransferase alpha | 1 |
| accD | acetyl-CoA carboxylase carboxyltransferase beta | 1 |
| lctP | L-lactate permease | 3 |
| ldh | L-lactate dehydrogenase | 2 |
| bcd | butyryl-CoA dehydrogenase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 2 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| ldhCLH_2335 | ACD51776.1 | OG0000131 | CP001078.1:2506027–2506977 - | L-lactate dehydrogenase [Clostridium botulinum E3 str. Alaska E43] |
| ldhCLH_3305 | ACD52185.1 | OG0000131 | CP001078.1:3574996–3575943 - | L-lactate dehydrogenase [Clostridium botulinum E3 str. Alaska E43] |
| hbdCLH_0365 | ACD52411.1 | OG0000229 | CP001078.1:380441–381289 + | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum E3 str. Alaska E43] |
| hbdCLH_1365 | ACD52176.1 | OG0000229 | CP001078.1:1460494–1461423 + | 3-hydroxybutyryl-coa dehydrogenase [Clostridium botulinum E3 str. Alaska E43] |
| thlCLH_0482 | ACD52338.1 | OG0000234 | CP001078.1:485874–487055 + | acetyl-CoA acetyltransferase [Clostridium botulinum E3 str. Alaska E43] |
| accDCLH_1089 | ACD52359.1 | OG0000403 | CP001078.1:1146624–1147499 + | acetyl-CoA carboxylase, carboxyl transferase, beta subunit [Clostridium botulinum E3 str. Alaska E43] |
| accACLH_1090 | ACD51908.1 | OG0000885 | CP001078.1:1147492–1148316 + | acetyl-CoA carboxylase, carboxyl transferase, alpha subunit [Clostridium botulinum E3 str. Alaska E43] |
| lctPCLH_0347 | ACD52377.1 | OG0001228 | CP001078.1:361901–362167 + | L-lactate permease [Clostridium botulinum E3 str. Alaska E43] |
| lctPCLH_0353 | ACD51371.1 | OG0001228 | CP001078.1:367280–367723 + | L-lactate permease [Clostridium botulinum E3 str. Alaska E43] |
| lctPCLH_1309 | ACD52673.1 | OG0001228 | CP001078.1:1388238–1389788 + | L-lactate permease [Clostridium botulinum E3 str. Alaska E43] |
| bcdCLH_0362 | ACD51748.1 | OG0001311 | CP001078.1:377368–378507 + | butyryl-CoA dehydrogenase [Clostridium botulinum E3 str. Alaska E43] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.