Assembly
ASM2034v1
BioProject PRJNA29077 · BioSample SAMN02603539
GENOME-RESOLVED EVIDENCE
GCA_000020345.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4257769 bp · GC 28.000%
Completeness 99.490% · contamination —%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 1/3 | 0.333 | Partial/absent |
| ethanol_entry_support | 0/1 | 0.000 | Partial/absent |
| fatty_acid_biosynthesis_core | 2/8 | 0.250 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 2/4 | 0.500 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| accA | acetyl-CoA carboxylase carboxyltransferase alpha | 1 |
| accD | acetyl-CoA carboxylase carboxyltransferase beta | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| bcd | butyryl-CoA dehydrogenase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| bcdCLJ_B3471 | ACQ54081.1 | OG0000034 | CP001083.1:3485931–3487070 - | butyryl-CoA dehydrogenase [Clostridium botulinum Ba4 str. 657] |
| ldhCLJ_B1617 | ACQ53261.1 | OG0000131 | CP001083.1:1650698–1651654 - | L-lactate dehydrogenase [Clostridium botulinum Ba4 str. 657] |
| hbdCLJ_B3473 | ACQ54363.1 | OG0000229 | CP001083.1:3488467–3489297 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum Ba4 str. 657] |
| accDCLJ_B3924 | ACQ54084.1 | OG0000403 | CP001083.1:3929934–3930803 - | acetyl-CoA carboxylase, carboxyl transferase, beta subunit [Clostridium botulinum Ba4 str. 657] |
| accACLJ_B3923 | ACQ54234.1 | OG0000885 | CP001083.1:3929048–3929887 - | acetyl-CoA carboxylase, carboxyl transferase, alpha subunit [Clostridium botulinum Ba4 str. 657] |
| lctPCLJ_B1050 | ACQ53803.1 | OG0001228 | CP001083.1:1069269–1070792 + | L-lactate permease [Clostridium botulinum Ba4 str. 657] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.