Assembly
ASM6358v1
BioProject PRJNA193 · BioSample SAMEA1705919
GENOME-RESOLVED EVIDENCE
GCA_000063585.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3903260 bp · GC 28.000%
Completeness 99.410% · contamination 0.170%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 1/3 | 0.333 | Partial/absent |
| ethanol_entry_support | 0/1 | 0.000 | Partial/absent |
| fatty_acid_biosynthesis_core | 0/8 | 0.000 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| bcd | butyryl-CoA dehydrogenase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
| coat | butyrate/acetoacetate CoA-transferase | 2 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| bcdCBO3199 | CAL84760.1 | OG0000034 | AM412317.1:3407583–3408722 - | butyryl-CoA dehydrogenase [Clostridium botulinum A str. ATCC 3502] |
| ldhCBO1519 | CAL83060.1 | OG0000131 | AM412317.1:1653007–1653963 - | L-lactate dehydrogenase [Clostridium botulinum A str. ATCC 3502] |
| ctfACBO2847 | CAL84410.1 | OG0000158 | AM412317.1:3024128–3024844 + | butyrate-acetoacetate CoA-transferase subunit A [Clostridium botulinum A str. ATCC 3502] |
| ctfBCBO2848 | CAL84411.1 | OG0000158 | AM412317.1:3024856–3025509 + | butyrate-acetoacetate CoA-transferase subunit B [Clostridium botulinum A str. ATCC 3502] |
| hbdCBO3201 | CAL84762.1 | OG0000229 | AM412317.1:3410119–3410949 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum A str. ATCC 3502] |
| thlCBO3200 | CAL84761.1 | OG0000234 | AM412317.1:3408916–3410109 - | acetyl-CoA acetyltransferase (thiolase) [Clostridium botulinum A str. ATCC 3502] |
| glcACBO1005 | CAL82558.1 | OG0001228 | AM412317.1:1106422–1107945 + | lactate permease [Clostridium botulinum A str. ATCC 3502] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.