Assembly
ASM21885v1
BioProject PRJNA63241 · BioSample SAMN02603396
GENOME-RESOLVED EVIDENCE
GCA_000218855.1
Clostridium acetobutylicum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4145581 bp · GC 31.000%
Completeness 98.050% · contamination 0.830%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 1/3 | 0.333 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 1/2 | 0.500 | Partial/absent |
| reverse_beta_oxidation_core | 4/4 | 1.000 | Complete marker set |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 2 |
| accD | acetyl-CoA carboxylase carboxyltransferase beta | 1 |
| fabG | 3-oxoacyl-ACP reductase | 1 |
| fabH | 3-oxoacyl-ACP synthase III | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| bcd | butyryl-CoA dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 3 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 2 |
| coat | butyrate/acetoacetate CoA-transferase | 2 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| ldh1SMB_G0272 | AEI34058.1 | OG0000131 | CP002660.1:298775–299716 + | L-lactate dehydrogenase [Clostridium acetobutylicum DSM 1731] |
| ctfaSMB_P161 | AEI34904.1 | OG0000158 | CP002661.1:178459–179115 + | butyrate-acetoacetate COA-transferase subunit A (plasmid) [Clostridium acetobutylicum DSM 1731] |
| ctfbSMB_P162 | AEI34905.1 | OG0000158 | CP002661.1:179117–179782 + | butyrate-acetoacetate COA-transferase subunit B (plasmid) [Clostridium acetobutylicum DSM 1731] |
| fabHSMB_G2043 | AEI34559.1 | OG0000222 | CP002660.1:2126003–2127139 - | 3-oxoacyl-ACP synthase III [Clostridium acetobutylicum DSM 1731] |
| hbdSMB_G2743 | AEI33291.1 | OG0000229 | CP002660.1:2833358–2834206 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium acetobutylicum DSM 1731] |
| thlSMB_G2909 | AEI32591.1 | OG0000234 | CP002660.1:3007590–3008768 - | acetyl-CoA acetyltransferase [Clostridium acetobutylicum DSM 1731] |
| thilSMB_P077 | AEI34820.1 | OG0000234 | CP002661.1:82040–83218 - | acetyl-CoA acetyltransferase (plasmid) [Clostridium acetobutylicum DSM 1731] |
| accDSMB_G3610 | AEI34012.1 | OG0000403 | CP002660.1:3771495–3772352 - | Acetyl-CoA carboxylase beta subunit [Clostridium acetobutylicum DSM 1731] |
| adhe1SMB_P160 | AEI34903.1 | OG0001124 | CP002661.1:175810–178398 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase (plasmid) [Clostridium acetobutylicum DSM 1731] |
| bcdSMB_G2746 | AEI33941.1 | OG0001311 | CP002660.1:2836138–2837277 - | butyryl-CoA dehydrogenase [Clostridium acetobutylicum DSM 1731] |
| fabGSMB_G3500 | AEI34322.1 | OG0004652 | CP002660.1:3663279–3664067 + | 3-oxoacyl-acyl carrier protein reductase [Clostridium acetobutylicum DSM 1731] |
| adheSMB_P034 | AEI34777.1 | OG0012903 | CP002661.1:33722–36298 - | bifunctional acetaldehyde-CoA/alcohol dehydrogenase (plasmid) [Clostridium acetobutylicum DSM 1731] |
| fadBSMB_G2044 | AEI34355.1 | OG0014900 | CP002660.1:2127141–2127830 - | Enoyl-CoA hydratase [Clostridium acetobutylicum DSM 1731] |
| fadJSMB_G2048 | AEI33740.1 | OG0014902 | CP002660.1:2129432–2130169 - | enoyl-CoA hydratase [Clostridium acetobutylicum DSM 1731] |
| fadBSMB_G0570 | AEI34354.1 | OG0018950 | CP002660.1:645595–645786 + | Enoyl-CoA hydratase [Clostridium acetobutylicum DSM 1731] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.