Assembly
ASM25319v1
BioProject PRJEA61511 · BioSample SAMEA2272270
GENOME-RESOLVED EVIDENCE
GCA_000253195.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3919740 bp · GC 28.000%
Completeness 95.310% · contamination 0.170%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 1/3 | 0.333 | Partial/absent |
| ethanol_entry_support | 0/1 | 0.000 | Partial/absent |
| fatty_acid_biosynthesis_core | 0/8 | 0.000 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 6/6 | 1.000 | Complete marker set |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| rnfB | Rnf complex subunit B | 1 |
| rnfC | Rnf complex subunit C | 1 |
| rnfD | Rnf complex subunit D | 1 |
| rnfE | Rnf complex subunit E | 1 |
| rnfG | Rnf complex subunit G | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 2 |
| bcd | butyryl-CoA dehydrogenase | 4 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
| coat | butyrate/acetoacetate CoA-transferase | 2 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| bcdH04402_02205 | CBZ04013.1 | OG0000034 | FR773526.1:2353533–2354666 - | butyryl-CoA dehydrogenase [Clostridium botulinum H04402 065] |
| bcdH04402_02923 | CBZ04727.1 | OG0000034 | FR773526.1:3040710–3042617 - | butyryl-CoA dehydrogenase [Clostridium botulinum H04402 065] |
| bcdH04402_03287 | CBZ05088.1 | OG0000034 | FR773526.1:3420867–3422006 - | butyryl-CoA dehydrogenase [Clostridium botulinum H04402 065] |
| bcdH04402_03373 | CBZ05173.1 | OG0000034 | FR773526.1:3507643–3508776 - | butyryl-CoA dehydrogenase [Clostridium botulinum H04402 065] |
| rnfCH04402_00380 | CBZ02196.1 | OG0000037 | FR773526.1:457162–458373 + | electron transport complex protein RnfC [Clostridium botulinum H04402 065] |
| ldhH04402_01586 | CBZ03398.1 | OG0000131 | FR773526.1:1711494–1712450 - | L-lactate dehydrogenase [Clostridium botulinum H04402 065] |
| coatH04402_02933 | CBZ04737.1 | OG0000158 | FR773526.1:3048324–3049040 + | butyrate-acetoacetate CoA-transferase subunit A [Clostridium botulinum H04402 065] |
| coatH04402_02934 | CBZ04738.1 | OG0000158 | FR773526.1:3049049–3049705 + | butyrate-acetoacetate CoA-transferase subunit B [Clostridium botulinum H04402 065] |
| hbdH04402_03289 | CBZ05090.1 | OG0000229 | FR773526.1:3423403–3424233 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum H04402 065] |
| thlH04402_03288 | CBZ05089.1 | OG0000234 | FR773526.1:3422200–3423378 - | acetyl-CoA acetyltransferase [Clostridium botulinum H04402 065] |
| lctPH04402_01078 | CBZ02893.1 | OG0001228 | FR773526.1:1168280–1169803 + | L-lactate permease [Clostridium botulinum H04402 065] |
| rnfGH04402_00382 | CBZ02198.1 | OG0001254 | FR773526.1:459328–459999 + | electron transport complex protein RnfG [Clostridium botulinum H04402 065] |
| rnfEH04402_00383 | CBZ02199.1 | OG0001320 | FR773526.1:460013–460612 + | electron transport complex protein RnfE [Clostridium botulinum H04402 065] |
| rnfAH04402_00384 | CBZ02200.1 | OG0001328 | FR773526.1:460665–461243 + | electron transport complex protein RnfA [Clostridium botulinum H04402 065] |
| rnfDH04402_00381 | CBZ02197.1 | OG0001342 | FR773526.1:458396–459328 + | electron transport complex protein RnfD [Clostridium botulinum H04402 065] |
| rnfBH04402_00385 | CBZ02201.1 | OG0001429 | FR773526.1:461257–462090 + | electron transport complex protein RnfB [Clostridium botulinum H04402 065] |
| ldhH04402_01230 | CBZ03045.1 | OG0003138 | FR773526.1:1334100–1334516 + | L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases [Clostridium botulinum H04402 065] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.