Assembly
ASM30694v1
BioProject PRJEB144 · BioSample SAMEA2272749
GENOME-RESOLVED EVIDENCE
Bordetella pertussis 18323 · Complete Genome
View versioned NCBI assembly →
ASM30694v1
BioProject PRJEB144 · BioSample SAMEA2272749
Genome size 4043846 bp · GC 67.500%
Completeness —% · contamination —%
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 0/1 | 0.000 | Partial/absent |
| fatty_acid_biosynthesis_core | 6/8 | 0.750 | Partial/absent |
| lactate_entry_support | 0/2 | 0.000 | Partial/absent |
| reverse_beta_oxidation_core | 2/4 | 0.500 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
| Marker | Function | Copies |
|---|
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| accAWP_003810007.1 · acetyl-CoA carboxylase carboxyltransferase subunit alpha | High | Approved candidatestrong profile and bridge orthogroup support | 346.200 / 0.98900 | Bridge orthogroup OG0001120 |
| accBWP_003814952.1 · acetyl-CoA carboxylase biotin carboxyl carrier protein | Medium | Approved candidatestrong profile plus independent annotation support: gene_symbol | 133.600 / 0.98100 | Annotation: gene_symbol |
| accBWP_004568140.1 · acetyl-CoA carboxylase biotin carboxyl carrier protein | Medium | Approved candidatestrong profile plus independent annotation support: gene_symbol | 107.900 / 0.97500 | Annotation: gene_symbol |
| accCWP_014905680.1 · acetyl-CoA carboxylase biotin carboxylase subunit | Medium | Approved candidatestrong profile plus independent annotation support: gene_symbol | 594.900 / 0.99100 | Annotation: gene_symbol |
| accCWP_003814951.1 · acetyl-CoA carboxylase biotin carboxylase subunit | Medium | Approved candidatestrong profile plus independent annotation support: gene_symbol | 577.200 / 0.99600 | Annotation: gene_symbol |
| accDWP_003813843.1 · acetyl-CoA carboxylase, carboxyltransferase subunit beta | High | Approved candidatestrong profile and bridge orthogroup support; annotation support: gene_symbol | 323.400 / 0.91100 | Bridge orthogroup OG0000844 |
| coatWP_003812478.1 · 3-oxoacid CoA-transferase subunit B | Medium | Approved candidatestrong profile plus independent annotation support: specific_product_name | 163.600 / 0.93500 | Annotation: specific_product_name |
| coatWP_003819086.1 · 3-oxoacid CoA-transferase subunit B | Medium | Approved candidatestrong profile plus independent annotation support: specific_product_name | 161.600 / 0.88900 | Annotation: specific_product_name |
| fabFWP_003813802.1 · beta-ketoacyl-ACP synthase II | Medium | Approved candidatestrong profile plus independent annotation support: gene_symbol | 633.700 / 0.99000 | Annotation: gene_symbol |
| fabGWP_003813819.1 · 3-oxoacyl-ACP reductase FabG | High | Approved candidatestrong profile and bridge orthogroup support; annotation support: gene_symbol | 243.200 / 0.98800 | Bridge orthogroup OG0000008 |
| fabIWP_003814739.1 · enoyl-ACP reductase FabI | Medium | Approved candidatestrong profile plus independent annotation support: gene_symbol | 378.500 / 0.97700 | Annotation: gene_symbol |
| fabIWP_014905656.1 · enoyl-ACP reductase FabI | Medium | Approved candidatestrong profile plus independent annotation support: gene_symbol | 307.500 / 0.96500 | Annotation: gene_symbol |
| fabZWP_014905606.1 · 3-hydroxyacyl-ACP dehydratase FabZ | High | Approved candidatestrong profile and bridge orthogroup support; annotation support: gene_symbol | 122.700 / 0.76000 | Bridge orthogroup OG0000074 |
| hbdWP_014905462.1 · 3-hydroxyacyl-CoA dehydrogenase family protein | High | Approved candidatestrong profile and bridge orthogroup support | 284.500 / 0.98600 | Bridge orthogroup OG0000153 |
| thlWP_014905810.1 · beta-ketothiolase BktB | High | Approved candidatestrong profile and bridge orthogroup support | 483.900 / 0.99700 | Bridge orthogroup OG0000010 |
| thlWP_014905519.1 · 3-oxoadipyl-CoA thiolase | High | Approved candidatestrong profile and bridge orthogroup support | 416.000 / 0.98700 | Bridge orthogroup OG0000010 |
| thlWP_003814674.1 · acetyl-CoA C-acyltransferase | High | Approved candidatestrong profile and bridge orthogroup support | 404.800 / 0.99000 | Bridge orthogroup OG0000010 |
| thlWP_003820178.1 · acetyl-CoA C-acyltransferase | High | Approved candidatestrong profile and bridge orthogroup support | 384.400 / 0.99200 | Bridge orthogroup OG0000010 |
| thlWP_014905840.1 · acetyl-CoA C-acyltransferase | High | Approved candidatestrong profile and bridge orthogroup support | 354.200 / 0.99000 | Bridge orthogroup OG0000010 |
| thlWP_010929948.1 · acetyl-CoA C-acyltransferase | High | Approved candidatestrong profile and bridge orthogroup support | 340.700 / 0.99500 | Bridge orthogroup OG0000010 |
| fabGWP_010931528.1 · SDR family oxidoreductase | Low | Excluded: low confidenceprofile hit lacks strong score and bridge orthogroup support | 91.800 / 0.78200 | Profile only |
| fabGWP_010930507.1 · SDR family oxidoreductase | Low | Excluded: low confidenceprofile hit lacks strong score and bridge orthogroup support | 88.400 / 0.93500 | Profile only |
| fabGWP_010926447.1 · SDR family oxidoreductase | Low | Excluded: low confidenceprofile hit lacks strong score and bridge orthogroup support | 83.200 / 0.77800 | Profile only |
| accCWP_014905468.1 · acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha | Medium | Needs reviewretain confidence tier and orthogroup-support fields during import | 508.400 / 0.99100 | Profile only |
| bcdWP_014905461.1 · acyl-CoA dehydrogenase family protein | Medium | Needs reviewretain confidence tier and orthogroup-support fields during import | 385.700 / 0.97900 | Profile only |
| bcdWP_014905608.1 · acyl-CoA dehydrogenase family protein | Medium | Needs reviewretain confidence tier and orthogroup-support fields during import | 363.400 / 0.99700 | Profile only |
| bcdWP_010929731.1 · acyl-CoA dehydrogenase family protein | Medium | Needs reviewretain confidence tier and orthogroup-support fields during import | 285.700 / 0.88400 | Profile only |
| bcdWP_010930413.1 · acyl-CoA dehydrogenase | Medium | Needs reviewretain confidence tier and orthogroup-support fields during import | 256.900 / 0.98400 | Profile only |
| crtWP_003815367.1 · enoyl-CoA hydratase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 269.500 / 0.97700 | Annotation: specific_product_name |
| crtWP_014905843.1 · crotonase/enoyl-CoA hydratase family protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 202.500 / 0.96900 | Annotation: specific_product_name |
| crtWP_010931604.1 · enoyl-CoA hydratase/isomerase family protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 190.200 / 0.98100 | Annotation: specific_product_name |
| crtWP_010929791.1 · crotonase/enoyl-CoA hydratase family protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 169.200 / 0.93800 | Annotation: specific_product_name |
| crtWP_010929943.1 · enoyl-CoA hydratase/isomerase family protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 166.600 / 0.96500 | Annotation: specific_product_name |
| crtWP_014905432.1 · enoyl-CoA hydratase/isomerase family protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 165.900 / 0.97300 | Annotation: specific_product_name |
| crtWP_080019429.1 · enoyl-CoA hydratase/isomerase family protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 164.400 / 0.95000 | Annotation: specific_product_name |
| crtWP_014905692.1 · enoyl-CoA hydratase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 163.500 / 0.96500 | Annotation: specific_product_name |
| crtWP_003816690.1 · enoyl-CoA hydratase/isomerase family protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 158.100 / 0.96900 | Annotation: specific_product_name |
| crtWP_010931073.1 · 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase PaaG | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 157.600 / 1.00000 | Profile only |
| crtWP_010930573.1 · enoyl-CoA hydratase/isomerase family protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 154.000 / 0.98100 | Annotation: specific_product_name |
| crtWP_010929730.1 · enoyl-CoA hydratase family protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 151.300 / 0.96100 | Annotation: specific_product_name |
| crtWP_010930516.1 · enoyl-CoA hydratase/isomerase family protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 149.900 / 0.94200 | Annotation: specific_product_name |
| crtWP_003817676.1 · enoyl-CoA hydratase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 143.400 / 0.82600 | Annotation: specific_product_name |
| crtWP_014486098.1 · enoyl-CoA hydratase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 141.700 / 0.96100 | Annotation: specific_product_name |
| crtWP_014906051.1 · 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 139.900 / 0.76400 | Profile only |
| crtWP_010929865.1 · enoyl-CoA hydratase/isomerase family protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 137.600 / 0.91500 | Annotation: specific_product_name |
| crtWP_003815315.1 · enoyl-CoA hydratase/isomerase family protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 128.400 / 0.94200 | Annotation: specific_product_name |
| crtWP_014905460.1 · enoyl-CoA hydratase-related protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 125.400 / 0.96100 | Annotation: specific_product_name |
| crtWP_014905841.1 · 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 124.500 / 0.73000 | Profile only |
| etfAWP_010930111.1 · electron transfer flavoprotein subunit alpha/FixB family protein | Medium | Needs reviewretain confidence tier and orthogroup-support fields during import | 229.000 / 0.94300 | Profile only |
| fabGWP_003820134.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 217.400 / 0.98800 | Profile only |
| fabGWP_003813586.1 · acetoacetyl-CoA reductase | High | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 216.900 / 0.98000 | Bridge orthogroup OG0000008 |
| fabGWP_010930042.1 · SDR family oxidoreductase | High | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 208.100 / 0.97600 | Bridge orthogroup OG0000008 |
| fabGWP_010931160.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 196.800 / 0.98400 | Profile only |
| fabGWP_041166302.1 · glucose 1-dehydrogenase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 196.200 / 0.98800 | Profile only |
| fabGWP_014905477.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 190.500 / 0.97600 | Profile only |
| fabGWP_003815002.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 189.500 / 0.98800 | Profile only |
| fabGWP_003818228.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 187.600 / 0.99200 | Profile only |
| fabGWP_010930206.1 · SDR family oxidoreductase | High | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 185.300 / 0.98000 | Bridge orthogroup OG0000008 |
| fabGWP_010930004.1 · SDR family oxidoreductase | High | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 182.300 / 0.97200 | Bridge orthogroup OG0000008 |
| fabGWP_014905442.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 181.700 / 0.92300 | Profile only |
| fabGWP_003814011.1 · 3-hydroxybutyrate dehydrogenase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 178.400 / 0.98800 | Profile only |
| fabGWP_014905729.1 · SDR family NAD(P)-dependent oxidoreductase | High | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 174.600 / 0.98400 | Bridge orthogroup OG0000008 |
| fabGWP_010930995.1 · SDR family oxidoreductase | High | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 174.500 / 0.98400 | Bridge orthogroup OG0000008 |
| fabGWP_014905845.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 164.800 / 0.98400 | Profile only |
| fabGWP_003810494.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 164.500 / 0.97200 | Profile only |
| fabGWP_019247851.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 162.900 / 0.97600 | Profile only |
| fabGWP_010929700.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 162.200 / 0.97600 | Profile only |
| fabGWP_010927020.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 160.200 / 0.96400 | Profile only |
| fabGWP_010926609.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 158.700 / 0.98800 | Profile only |
| fabGWP_003816687.1 · SDR family NAD(P)-dependent oxidoreductase | High | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 154.900 / 0.96000 | Bridge orthogroup OG0000008 |
| fabGWP_010929694.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 154.600 / 0.95600 | Profile only |
| fabGWP_003811135.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 145.100 / 0.92300 | Profile only |
| fabGWP_010931126.1 · 3-hydroxyacyl-CoA dehydrogenase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 140.400 / 0.98800 | Profile only |
| fabGWP_003813618.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 139.800 / 0.95200 | Profile only |
| fabGWP_003819553.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 136.900 / 0.97600 | Profile only |
| fabGWP_014905527.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 117.200 / 0.97200 | Profile only |
| fabGWP_014486095.1 · short-chain dehydrogenase/reductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 116.200 / 0.98400 | Profile only |
| fabGWP_003813987.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 106.400 / 0.98000 | Profile only |
| fabHWP_010930935.1 · beta-ketoacyl-ACP synthase III | Medium | Needs reviewno bridge-supported orthogroup; require domain, motif, or neighborhood confirmation | 288.400 / 0.98800 | Profile only |
| fabLWP_041166302.1 · glucose 1-dehydrogenase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 227.800 / 0.98800 | Profile only |
| fabLWP_003820134.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 208.200 / 0.98800 | Profile only |
| fabLWP_014905477.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 205.700 / 0.98000 | Profile only |
| fabLWP_010931160.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 205.500 / 0.98000 | Profile only |
| fabLWP_003818228.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 204.500 / 0.97200 | Profile only |
| fabLWP_003813819.1 · 3-oxoacyl-ACP reductase FabG | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 191.400 / 0.98000 | Profile only |
| fabLWP_010929700.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 190.700 / 0.97600 | Profile only |
| fabLWP_014905442.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 189.400 / 0.91700 | Profile only |
| fabLWP_010930042.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 183.100 / 0.97600 | Profile only |
| fabLWP_003815002.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 174.900 / 0.98000 | Profile only |
| fabLWP_010931528.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 172.100 / 0.92100 | Profile only |
| fabLWP_010926609.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 169.000 / 0.99200 | Profile only |
| fabLWP_019247851.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 166.300 / 0.97600 | Profile only |
| fabLWP_003814011.1 · 3-hydroxybutyrate dehydrogenase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 165.700 / 0.97600 | Profile only |
| fabLWP_010930995.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 159.800 / 0.98800 | Profile only |
| fabLWP_010930206.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 158.000 / 0.98400 | Profile only |
| fabLWP_010930004.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 156.000 / 0.98000 | Profile only |
| fabLWP_010927020.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 152.900 / 0.96400 | Profile only |
| fabLWP_003813586.1 · acetoacetyl-CoA reductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 151.900 / 0.97600 | Profile only |
| fabLWP_003810494.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 147.500 / 0.97600 | Profile only |
| fabLWP_003819553.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 146.800 / 0.98800 | Profile only |
| fabLWP_014905845.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 145.500 / 0.97200 | Profile only |
| fabLWP_003811135.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 139.300 / 0.88500 | Profile only |
| fabLWP_014905729.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 135.300 / 0.98400 | Profile only |
| fabLWP_003813618.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 132.600 / 0.98400 | Profile only |
| fabLWP_014905527.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 132.300 / 0.98800 | Profile only |
| fabLWP_003816687.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 128.500 / 0.96000 | Profile only |
| fabLWP_010929694.1 · SDR family NAD(P)-dependent oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 128.400 / 0.96400 | Profile only |
| fabLWP_014486095.1 · short-chain dehydrogenase/reductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 124.000 / 0.98000 | Profile only |
| fabLWP_010930507.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 121.800 / 0.94000 | Profile only |
| fabLWP_003813987.1 · SDR family oxidoreductase | Medium | Needs reviewextreme per-proteome copy number; profile alone is not function-specific | 116.300 / 0.97200 | Profile only |
| hbdWP_014906051.1 · 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein | Medium | Needs reviewretain confidence tier and orthogroup-support fields during import | 212.700 / 0.97500 | Profile only |
| hbdWP_014905841.1 · 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein | Medium | Needs reviewretain confidence tier and orthogroup-support fields during import | 201.200 / 0.98600 | Profile only |
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.