Assembly
ASM30712v1
BioProject PRJEA60761 · BioSample SAMEA2272780
GENOME-RESOLVED EVIDENCE
GCA_000307125.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3829198 bp · GC 27.500%
Completeness 98.940% · contamination —%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 1/3 | 0.333 | Partial/absent |
| ethanol_entry_support | 0/1 | 0.000 | Partial/absent |
| fatty_acid_biosynthesis_core | 0/8 | 0.000 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 2 |
| bcd | butyryl-CoA dehydrogenase | 3 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
| coat | butyrate/acetoacetate CoA-transferase | 4 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| bcdCB17B1292 | CDH90281.1 | OG0000034 | FR745875.1:1400655–1401794 + | Butyryl-CoA dehydrogenase [Clostridium botulinum B str. Eklund 17B (NRP)] |
| bcdCB17B1909 | CDH90898.1 | OG0000034 | FR745875.1:2066676–2067815 - | Butyryl-CoA dehydrogenase [Clostridium botulinum B str. Eklund 17B (NRP)] |
| ldhCB17B2483 | CDH91472.1 | OG0000131 | FR745875.1:2678755–2679705 - | L-lactate dehydrogenase [Clostridium botulinum B str. Eklund 17B (NRP)] |
| ldhCB17B3390 | CDH92379.1 | OG0000131 | FR745875.1:3698707–3699654 - | L-lactate dehydrogenase [Clostridium botulinum B str. Eklund 17B (NRP)] |
| ctfACB17B0630 | CDH89620.1 | OG0000158 | FR745875.1:700148–700900 + | butyrate-acetoacetate CoA-transferase subunit A [Clostridium botulinum B str. Eklund 17B (NRP)] |
| ctfBCB17B0631 | CDH89621.1 | OG0000158 | FR745875.1:700913–701566 + | butyrate-acetoacetate CoA-transferase subunit B [Clostridium botulinum B str. Eklund 17B (NRP)] |
| coatCB17B2049 | CDH91038.1 | OG0000158 | FR745875.1:2231603–2232268 - | Butyrate-acetoacetate CoA-transferase subunit B [Clostridium botulinum B str. Eklund 17B (NRP)] |
| coatCB17B2050 | CDH91039.1 | OG0000158 | FR745875.1:2232269–2232922 - | Butyrate-acetoacetate CoA-transferase subunit A [Clostridium botulinum B str. Eklund 17B (NRP)] |
| hbdCB17B0345 | CDH89337.1 | OG0000229 | FR745875.1:384846–385733 + | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum B str. Eklund 17B (NRP)] |
| thlCB17B0436 | CDH89427.1 | OG0000234 | FR745875.1:490946–492127 + | acetyl-CoA acetyltransferase (thiolase) [Clostridium botulinum B str. Eklund 17B (NRP)] |
| glcACB17B1290 | CDH90279.1 | OG0001228 | FR745875.1:1397340–1398890 + | lactate permease [Clostridium botulinum B str. Eklund 17B (NRP)] |
| bcdCB17B0342 | CDH89334.1 | OG0001311 | FR745875.1:381811–382950 + | butyryl-CoA dehydrogenase [Clostridium botulinum B str. Eklund 17B (NRP)] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.