Assembly
ASM81694v1
BioProject PRJNA222587 · BioSample SAMN03222819
GENOME-RESOLVED EVIDENCE
GCA_000816945.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3892029 bp · GC 28.000%
Completeness 99.450% · contamination 0.170%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 0/1 | 0.000 | Partial/absent |
| fatty_acid_biosynthesis_core | 2/8 | 0.250 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 2/4 | 0.500 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| accA | acetyl-CoA carboxylase carboxyltransferase alpha | 1 |
| accD | acetyl-CoA carboxylase carboxyltransferase beta | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| ldhT257_176 | AJD27230.1 | OG0000131 | CP006907.1:210274–211230 + | L-lactate dehydrogenase [Clostridium botulinum CDC_297] |
| thlAT257_2215 | AJD28361.1 | OG0000234 | CP006907.1:2340665–2341843 + | acetyl-CoA acetyltransferase [Clostridium botulinum CDC_297] |
| accDT257_1792 | AJD28438.1 | OG0000403 | CP006907.1:1903923–1904792 + | acetyl-CoA carboxylase, carboxyl transferase, beta subunit [Clostridium botulinum CDC_297] |
| accAT257_1793 | AJD26264.1 | OG0000885 | CP006907.1:1904839–1905678 + | acetyl-CoA carboxylase, carboxyl transferase, alpha subunit [Clostridium botulinum CDC_297] |
| lctPT257_735 | AJD25514.1 | OG0001228 | CP006907.1:795830–797353 - | transporter, lactate permease family protein [Clostridium botulinum CDC_297] |
| crtT257_2213 | AJD26278.1 | OG0001639 | CP006907.1:2339008–2339790 + | enoyl-CoA hydratase/isomerase family protein [Clostridium botulinum CDC_297] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.