Assembly
ASM82901v1
BioProject PRJDB3402 · BioSample SAMD00024166
GENOME-RESOLVED EVIDENCE
GCA_000829015.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3901300 bp · GC 28.500%
Completeness 99.490% · contamination 0.170%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 1/3 | 0.333 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accA | acetyl-CoA carboxylase carboxyltransferase alpha | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accD | acetyl-CoA carboxylase carboxyltransferase beta | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| bcd | butyryl-CoA dehydrogenase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| bcdCBB2_2744 | BAQ14854.1 | OG0000034 | AP014696.1:3429589–3430728 - | putative butyryl-CoA dehydrogenase [Clostridium botulinum] |
| ldhCBB2_1362 | BAQ13472.1 | OG0000131 | AP014696.1:1697732–1698688 - | putative L-lactate dehydrogenase [Clostridium botulinum] |
| accBCBB2_3092 | BAQ15202.1 | OG0000212 | AP014696.1:3856415–3856891 - | putative acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdCBB2_2746 | BAQ14856.1 | OG0000229 | AP014696.1:3432125–3432955 - | putative 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlCBB2_2745 | BAQ14855.1 | OG0000234 | AP014696.1:3430922–3432100 - | putative acetyl-CoA acetyltransferase [Clostridium botulinum] |
| accDCBB2_3089 | BAQ15199.1 | OG0000403 | AP014696.1:3853694–3854563 - | putative acetyl-CoA carboxylase carboxyl transferase subunit beta [Clostridium botulinum] |
| accACBB2_3088 | BAQ15198.1 | OG0000885 | AP014696.1:3852808–3853647 - | putative acetyl-CoA carboxylase carboxyl transferase subunit alpha [Clostridium botulinum] |
| adhECBB2_0323 | BAQ12433.1 | OG0001124 | AP014696.1:420303–422891 + | putative bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPCBB2_0920 | BAQ13030.1 | OG0001228 | AP014696.1:1142845–1144368 + | putative L-lactate permease [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.