Assembly
ASM188936v1
BioProject PRJNA301977 · BioSample SAMN04262332
GENOME-RESOLVED EVIDENCE
GCA_001889365.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4432564 bp · GC 28.000%
Completeness 98.730% · contamination 1.870%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 0/1 | 0.000 | Partial/absent |
| fatty_acid_biosynthesis_core | 2/8 | 0.250 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 2/4 | 0.500 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| accA | acetyl-CoA carboxylase carboxyltransferase alpha | 1 |
| accD | acetyl-CoA carboxylase carboxyltransferase beta | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| ldhNPD1_2982 | APH24937.1 | OG0000131 | CP013296.1:3072039–3072995 - | L-lactate dehydrogenase [Clostridium botulinum] |
| thlANPD1_690 | APH21487.1 | OG0000234 | CP013296.1:720960–722138 - | acetyl-CoA acetyltransferase [Clostridium botulinum] |
| accDNPD1_1217 | APH23811.1 | OG0000403 | CP013296.1:1221822–1222691 - | acetyl-CoA carboxylase, carboxyl transferase, beta subunit [Clostridium botulinum] |
| accANPD1_1216 | APH22200.1 | OG0000885 | CP013296.1:1220936–1221775 - | acetyl-CoA carboxylase, carboxyl transferase, alpha subunit [Clostridium botulinum] |
| lctPNPD1_2383 | APH23924.1 | OG0001228 | CP013296.1:2473714–2475237 + | transporter, lactate permease family protein [Clostridium botulinum] |
| crtNPD1_692 | APH22960.1 | OG0001639 | CP013296.1:723024–723806 - | enoyl-CoA hydratase/isomerase family protein [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.