Assembly
ASM286582v1
BioProject PRJNA302053 · BioSample SAMN04263501
GENOME-RESOLVED EVIDENCE
GCA_002865825.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4154949 bp · GC 28.000%
Completeness 98.980% · contamination 0.900%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 4/8 | 0.500 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| accD | acetyl-CoA carboxylase carboxyltransferase beta | 1 |
| fabG | 3-oxoacyl-ACP reductase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 3 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCRSJ19_19955 | AUN05044.1 | OG0000061 | CP013845.1:3876039–3877385 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZRSJ19_12560 | AUN03693.1 | OG0000119 | CP013845.1:2359884–2361575 + | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhRSJ19_09200 | AUN03085.1 | OG0000131 | CP013845.1:1647512–1648468 - | L-lactate dehydrogenase [Clostridium botulinum] |
| hbdRSJ19_17625 | AUN04634.1 | OG0000229 | CP013845.1:3411739–3412569 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlRSJ19_17620 | AUN04633.1 | OG0000234 | CP013845.1:3410536–3411714 - | acetyl-CoA acetyltransferase [Clostridium botulinum] |
| accDRSJ19_19950 | AUN05043.1 | OG0000403 | CP013845.1:3875150–3876019 - | acetyl-CoA carboxylase carboxyl transferase subunit beta [Clostridium botulinum] |
| fabGRSJ19_15005 | AUN04142.1 | OG0000440 | CP013845.1:2838089–2838943 - | 3-oxoacyl-ACP reductase [Clostridium botulinum] |
| adhERSJ19_03305 | AUN01996.1 | OG0001124 | CP013845.1:395960–398548 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| fabZRSJ19_17850 | AUN04679.1 | OG0001208 | CP013845.1:3455343–3456602 - | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| lctPRSJ19_06650 | AUN02606.1 | OG0001228 | CP013845.1:1102213–1103736 + | lactate permease [Clostridium botulinum] |
| crtRSJ19_17630 | AUN04635.1 | OG0001639 | CP013845.1:3412589–3413371 - | crotonase [Clostridium botulinum] |
| fabZRSJ19_12555 | AUN03692.1 | OG0004330 | CP013845.1:2358610–2359881 + | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.