Assembly
ASM301361v1
BioProject PRJNA431675 · BioSample SAMN08397864
GENOME-RESOLVED EVIDENCE
GCA_003013615.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4167815 bp · GC 28.000%
Completeness 98.780% · contamination 2.990%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 1/2 | 0.500 | Partial/absent |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCC3B64_15675 | AVP65604.1 | OG0000061 | CP027776.1:3282823–3284169 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZC3B64_05740 | AVP63785.1 | OG0000119 | CP027776.1:1248164–1249858 + | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhC3B64_03105 | AVP63300.1 | OG0000131 | CP027776.1:671823–672779 - | L-lactate dehydrogenase [Clostridium botulinum] |
| accBC3B64_15685 | AVP65606.1 | OG0000212 | CP027776.1:3284654–3285130 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdC3B64_16535 | AVP65760.1 | OG0000229 | CP027776.1:3454309–3455139 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlC3B64_16530 | AVP65759.1 | OG0000234 | CP027776.1:3453106–3454284 - | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| adhEC3B64_00375 | AVP62790.1 | OG0001124 | CP027776.1:94851–97439 - | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| rnfAC3B64_00220 | AVP62761.1 | OG0001328 | CP027776.1:58820–59398 - | electron transport complex protein RnfA [Clostridium botulinum] |
| crtC3B64_16540 | AVP65761.1 | OG0001639 | CP027776.1:3455159–3455941 - | crotonase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.