Assembly
ASM301365v1
BioProject PRJNA430768 · BioSample SAMN08394865
GENOME-RESOLVED EVIDENCE
GCA_003013655.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4005274 bp · GC 28.000%
Completeness 98.480% · contamination 2.860%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 1/2 | 0.500 | Partial/absent |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCC7M79_13020 | AVP61562.1 | OG0000061 | CP027775.1:2807600–2808946 + | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZC7M79_06525 | AVP60379.1 | OG0000119 | CP027775.1:1433596–1435290 + | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhC7M79_00940 | AVP59343.1 | OG0000131 | CP027775.1:221348–222304 + | L-lactate dehydrogenase [Clostridium botulinum] |
| accBC7M79_13010 | AVP61560.1 | OG0000212 | CP027775.1:2806639–2807115 + | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdC7M79_15495 | AVP62016.1 | OG0000229 | CP027775.1:3279452–3280282 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlC7M79_15490 | AVP62015.1 | OG0000234 | CP027775.1:3278249–3279427 - | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| adhEC7M79_08025 | AVP60652.1 | OG0001124 | CP027775.1:1761339–1763927 - | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| rnfAC7M79_07870 | AVP60623.1 | OG0001328 | CP027775.1:1725553–1726131 - | electron transport complex protein RnfA [Clostridium botulinum] |
| crtC7M79_15500 | AVP62017.1 | OG0001639 | CP027775.1:3280302–3281084 - | crotonase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.