Assembly
ASM301714v1
BioProject PRJNA431675 · BioSample SAMN08397979
GENOME-RESOLVED EVIDENCE
GCA_003017145.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3988693 bp · GC 28.000%
Completeness 99.490% · contamination 1.180%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 1/2 | 0.500 | Partial/absent |
| reverse_beta_oxidation_core | 2/4 | 0.500 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 2 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCC7M56_15440 | AVQ39999.1 | OG0000061 | CP027777.1:3287790–3289136 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZC7M56_10195 | AVQ39037.1 | OG0000119 | CP027777.1:2176167–2177861 - | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhC7M56_07765 | AVQ38581.1 | OG0000131 | CP027777.1:1680090–1681046 + | L-lactate dehydrogenase [Clostridium botulinum] |
| accBC7M56_15450 | AVQ40001.1 | OG0000212 | CP027777.1:3289622–3290098 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdC7M56_17425 | AVQ40370.1 | OG0000229 | CP027777.1:3703406–3704236 + | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| adhEC7M56_03900 | AVQ37865.1 | OG0001124 | CP027777.1:842000–844588 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| fabZC7M56_17210 | AVQ40327.1 | OG0001208 | CP027777.1:3660802–3662061 + | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| rnfAC7M56_04045 | AVQ37893.1 | OG0001328 | CP027777.1:880399–880977 + | electron transport complex protein RnfA [Clostridium botulinum] |
| crtC7M56_17420 | AVQ40369.1 | OG0001639 | CP027777.1:3702604–3703386 + | crotonase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.