Assembly
ASM301719v1
BioProject PRJNA431675 · BioSample SAMN08398059
GENOME-RESOLVED EVIDENCE
GCA_003017195.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4004635 bp · GC 28.000%
Completeness 99.490% · contamination 1.180%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 1/2 | 0.500 | Partial/absent |
| reverse_beta_oxidation_core | 2/4 | 0.500 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 2 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCC7M57_15615 | AVQ43600.1 | OG0000061 | CP027778.1:3326835–3328181 - | UNVERIFIED_CONTAM: acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZC7M57_09665 | AVQ42497.1 | OG0000119 | CP027778.1:2066339–2068033 - | UNVERIFIED_CONTAM: 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhC7M57_07235 | AVQ42041.1 | OG0000131 | CP027778.1:1571007–1571963 + | UNVERIFIED_CONTAM: L-lactate dehydrogenase [Clostridium botulinum] |
| accBC7M57_15625 | AVQ43602.1 | OG0000212 | CP027778.1:3328667–3329143 - | UNVERIFIED_CONTAM: acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdC7M57_17385 | AVQ43924.1 | OG0000229 | CP027778.1:3695654–3696484 + | UNVERIFIED_CONTAM: 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| adhEC7M57_02545 | AVQ41176.1 | OG0001124 | CP027778.1:581003–583591 - | UNVERIFIED_CONTAM: bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| fabZC7M57_17170 | AVQ43881.1 | OG0001208 | CP027778.1:3653050–3654309 + | UNVERIFIED_CONTAM: 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| rnfAC7M57_02400 | AVQ41148.1 | OG0001328 | CP027778.1:544614–545192 - | UNVERIFIED_CONTAM: electron transport complex protein RnfA [Clostridium botulinum] |
| crtC7M57_17380 | AVQ43923.1 | OG0001639 | CP027778.1:3694852–3695634 + | UNVERIFIED_CONTAM: crotonase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.