Assembly
ASM301722v1
BioProject PRJNA431675 · BioSample SAMN08398061
GENOME-RESOLVED EVIDENCE
GCA_003017225.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3998747 bp · GC 28.000%
Completeness 98.980% · contamination 0.660%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 5/8 | 0.625 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accA | acetyl-CoA carboxylase carboxyltransferase alpha | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabG | 3-oxoacyl-ACP reductase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 2 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCC7M58_14465 | AVQ50469.1 | OG0000061 | CP027779.1:3094690–3096036 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZC7M58_12495 | AVQ50105.1 | OG0000119 | CP027779.1:2656160–2657854 - | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhC7M58_07485 | AVQ49189.1 | OG0000131 | CP027779.1:1595087–1596043 - | L-lactate dehydrogenase [Clostridium botulinum] |
| accBC7M58_14475 | AVQ50471.1 | OG0000212 | CP027779.1:3096522–3096998 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdC7M58_17195 | AVQ50958.1 | OG0000229 | CP027779.1:3652384–3653214 + | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlC7M58_17200 | AVQ50959.1 | OG0000234 | CP027779.1:3653239–3654417 + | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| fabGC7M58_00755 | AVQ47940.1 | OG0000440 | CP027779.1:150850–151704 - | 3-oxoacyl-ACP reductase [Clostridium botulinum] |
| accAC7M58_14455 | AVQ50467.1 | OG0000885 | CP027779.1:3092915–3093754 - | acetyl-CoA carboxylase carboxyl transferase subunit alpha [Clostridium botulinum] |
| adhEC7M58_12955 | AVQ50193.1 | OG0001124 | CP027779.1:2762891–2765479 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| fabZC7M58_16980 | AVQ50915.1 | OG0001208 | CP027779.1:3609564–3610823 + | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| lctPC7M58_02290 | AVQ48226.1 | OG0001228 | CP027779.1:503391–504914 - | lactate permease [Clostridium botulinum] |
| rnfAC7M58_13110 | AVQ50222.1 | OG0001328 | CP027779.1:2802643–2803221 + | electron transport complex protein RnfA [Clostridium botulinum] |
| crtC7M58_17190 | AVQ50957.1 | OG0001639 | CP027779.1:3651582–3652364 + | crotonase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.