Assembly
ASM301726v1
BioProject PRJNA431675 · BioSample SAMN08398242
GENOME-RESOLVED EVIDENCE
GCA_003017265.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4002680 bp · GC 28.000%
Completeness —% · contamination —%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 5/8 | 0.625 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accA | acetyl-CoA carboxylase carboxyltransferase alpha | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabG | 3-oxoacyl-ACP reductase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 2 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCC7M60_11225 | AVQ46322.1 | OG0000061 | CP027781.1:2390497–2391843 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZC7M60_12230 | AVQ46499.1 | OG0000119 | CP027781.1:2596659–2598353 - | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhC7M60_05910 | AVQ45356.1 | OG0000131 | CP027781.1:1267386–1268342 - | L-lactate dehydrogenase [Clostridium botulinum] |
| accBC7M60_11235 | AVQ46324.1 | OG0000212 | CP027781.1:2392329–2392805 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdC7M60_16975 | AVQ47371.1 | OG0000229 | CP027781.1:3601384–3602214 + | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlC7M60_16980 | AVQ47372.1 | OG0000234 | CP027781.1:3602239–3603417 + | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| fabGC7M60_00760 | AVQ44397.1 | OG0000440 | CP027781.1:150611–151465 - | 3-oxoacyl-ACP reductase [Clostridium botulinum] |
| accAC7M60_11215 | AVQ46320.1 | OG0000885 | CP027781.1:2388722–2389561 - | acetyl-CoA carboxylase carboxyl transferase subunit alpha [Clostridium botulinum] |
| adhEC7M60_13345 | AVQ46707.1 | OG0001124 | CP027781.1:2831861–2834449 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| fabZC7M60_16760 | AVQ47328.1 | OG0001208 | CP027781.1:3558564–3559823 + | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| lctPC7M60_02295 | AVQ44682.1 | OG0001228 | CP027781.1:503153–504676 - | lactate permease [Clostridium botulinum] |
| rnfAC7M60_13500 | AVQ46735.1 | OG0001328 | CP027781.1:2871608–2872186 + | electron transport complex protein RnfA [Clostridium botulinum] |
| crtC7M60_16970 | AVQ47370.1 | OG0001639 | CP027781.1:3600582–3601364 + | crotonase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.