Assembly
ASM301733v1
BioProject PRJNA431675 · BioSample SAMN08398062
GENOME-RESOLVED EVIDENCE
GCA_003017335.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3978960 bp · GC 28.000%
Completeness 94.540% · contamination 0.750%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 1/8 | 0.125 | Partial/absent |
| lactate_entry_support | 1/2 | 0.500 | Partial/absent |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| fabZC7M59_11510 | AVQ53454.1 | OG0000119 | CP027780.1:2472950–2474644 + | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhC7M59_08300 | AVQ52858.1 | OG0000131 | CP027780.1:1755997–1756953 - | L-lactate dehydrogenase [Clostridium botulinum] |
| hbdC7M59_16815 | AVQ54431.1 | OG0000229 | CP027780.1:3561028–3561858 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlC7M59_16810 | AVQ54430.1 | OG0000234 | CP027780.1:3559825–3561003 - | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| adhEC7M59_01865 | AVQ51670.1 | OG0001124 | CP027780.1:387289–389877 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| crtC7M59_16820 | AVQ54432.1 | OG0001639 | CP027780.1:3561878–3562660 - | crotonase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.