Assembly
ASM305834v1
BioProject PRJNA290487 · BioSample SAMN03780372
GENOME-RESOLVED EVIDENCE
GCA_003058345.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3858511 bp · GC 28.000%
Completeness 99.070% · contamination 0.170%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 5/8 | 0.625 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| accD | acetyl-CoA carboxylase carboxyltransferase beta | 1 |
| fabH | 3-oxoacyl-ACP synthase III | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCDB732_18600 | AWB19399.1 | OG0000061 | CP028842.1:3811365–3812711 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZDB732_11440 | AWB18065.1 | OG0000119 | CP028842.1:2358402–2360096 + | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhDB732_08350 | AWB17478.1 | OG0000131 | CP028842.1:1698794–1699750 - | L-lactate dehydrogenase [Clostridium botulinum] |
| accBDB732_18610 | AWB19401.1 | OG0000212 | CP028842.1:3813197–3813673 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| fabHDB732_18640 | AWB19407.1 | OG0000222 | CP028842.1:3817981–3818961 - | 3-oxoacyl-ACP synthase III [Clostridium botulinum] |
| hbdDB732_16390 | AWB19011.1 | OG0000229 | CP028842.1:3381170–3382000 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlDB732_16385 | AWB19010.1 | OG0000234 | CP028842.1:3379967–3381145 - | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| accDDB732_18595 | AWB19398.1 | OG0000403 | CP028842.1:3810476–3811345 - | acetyl-CoA carboxylase carboxyl transferase subunit beta [Clostridium botulinum] |
| adhEDB732_01935 | AWB16259.1 | OG0001124 | CP028842.1:399593–402181 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPDB732_05485 | AWB16923.1 | OG0001228 | CP028842.1:1115338–1116861 + | L-lactate permease [Clostridium botulinum] |
| rnfADB732_02070 | AWB16285.1 | OG0001328 | CP028842.1:432769–433347 + | electron transport complex protein RnfA [Clostridium botulinum] |
| crtDB732_16395 | AWB19012.1 | OG0001639 | CP028842.1:3382031–3382813 - | crotonase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.