Assembly
ASM305844v1
BioProject PRJNA290487 · BioSample SAMN07135561
GENOME-RESOLVED EVIDENCE
GCA_003058445.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3930410 bp · GC 28.000%
Completeness 98.570% · contamination 0.170%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 5/8 | 0.625 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| accD | acetyl-CoA carboxylase carboxyltransferase beta | 1 |
| fabH | 3-oxoacyl-ACP synthase III | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCDBN47_18940 | AWB32217.1 | OG0000061 | CP028859.1:3873170–3874516 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZDBN47_11430 | AWB30843.1 | OG0000119 | CP028859.1:2358572–2360266 + | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhDBN47_08320 | AWB30266.1 | OG0000131 | CP028859.1:1692265–1693221 - | L-lactate dehydrogenase [Clostridium botulinum] |
| accBDBN47_18950 | AWB32219.1 | OG0000212 | CP028859.1:3875002–3875478 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| fabHDBN47_18980 | AWB32225.1 | OG0000222 | CP028859.1:3879786–3880766 - | 3-oxoacyl-ACP synthase III [Clostridium botulinum] |
| hbdDBN47_16685 | AWB31824.1 | OG0000229 | CP028859.1:3425891–3426721 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlDBN47_16680 | AWB31823.1 | OG0000234 | CP028859.1:3424688–3425866 - | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| accDDBN47_18935 | AWB32216.1 | OG0000403 | CP028859.1:3872281–3873150 - | acetyl-CoA carboxylase carboxyl transferase subunit beta [Clostridium botulinum] |
| adhEDBN47_01935 | AWB29076.1 | OG0001124 | CP028859.1:399574–402162 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPDBN47_05465 | AWB29720.1 | OG0001228 | CP028859.1:1112441–1113964 + | L-lactate permease [Clostridium botulinum] |
| rnfADBN47_02070 | AWB29102.1 | OG0001328 | CP028859.1:432749–433327 + | electron transport complex protein RnfA [Clostridium botulinum] |
| crtDBN47_16690 | AWB31825.1 | OG0001639 | CP028859.1:3426752–3427534 - | crotonase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.