Assembly
ASM334531v1
BioProject PRJNA243331 · BioSample SAMN03769222
GENOME-RESOLVED EVIDENCE
GCA_003345315.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4018503 bp · GC 28.000%
Completeness 99.490% · contamination 0.850%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 4/8 | 0.500 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabH | 3-oxoacyl-ACP synthase III | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCAGE31_13980 | AXG96683.1 | OG0000061 | CP031098.1:2877008–2878354 + | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZAGE31_02175 | AXG94547.1 | OG0000119 | CP031098.1:429336–431030 - | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhAGE31_05155 | AXG95088.1 | OG0000131 | CP031098.1:1067212–1068168 + | L-lactate dehydrogenase [Clostridium botulinum] |
| accBAGE31_13970 | AXG96681.1 | OG0000212 | CP031098.1:2876046–2876522 + | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| fabHAGE31_13940 | AXG96675.1 | OG0000222 | CP031098.1:2870758–2871738 + | 3-oxoacyl-ACP synthase III [Clostridium botulinum] |
| hbdAGE31_16335 | AXG97095.1 | OG0000229 | CP031098.1:3336727–3337557 + | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlAGE31_16340 | AXG97096.1 | OG0000234 | CP031098.1:3337582–3338760 + | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| adhEAGE31_11715 | AXG96293.1 | OG0001124 | CP031098.1:2427358–2429946 - | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPAGE31_08095 | AXG95645.1 | OG0001228 | CP031098.1:1667182–1668705 - | L-lactate permease [Clostridium botulinum] |
| rnfAAGE31_11575 | AXG96267.1 | OG0001328 | CP031098.1:2395908–2396486 - | electron transport complex protein RnfA [Clostridium botulinum] |
| crtAGE31_16330 | AXG97094.1 | OG0001639 | CP031098.1:3335914–3336696 + | crotonase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.