Assembly
ASM334533v1
BioProject PRJNA243331 · BioSample SAMN03769220
GENOME-RESOLVED EVIDENCE
GCA_003345335.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4202171 bp · GC 28.000%
Completeness 99.430% · contamination —%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 5/8 | 0.625 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| accD | acetyl-CoA carboxylase carboxyltransferase beta | 1 |
| fabG | 3-oxoacyl-ACP reductase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCAGE29_14000 | AXG92800.1 | OG0000061 | CP031097.1:2515595–2516941 + | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZAGE29_02165 | AXG90636.1 | OG0000119 | CP031097.1:121062–122753 - | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhAGE29_05595 | AXG91266.1 | OG0000131 | CP031097.1:819286–820242 + | L-lactate dehydrogenase [Clostridium botulinum] |
| accBAGE29_13990 | AXG92798.1 | OG0000212 | CP031097.1:2514633–2515109 + | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdAGE29_16295 | AXG93195.1 | OG0000229 | CP031097.1:2963693–2964523 + | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlAGE29_16300 | AXG93196.1 | OG0000234 | CP031097.1:2964548–2965726 + | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| accDAGE29_14005 | AXG92801.1 | OG0000403 | CP031097.1:2516961–2517830 + | acetyl-CoA carboxylase carboxyl transferase subunit beta [Clostridium botulinum] |
| fabGAGE29_19000 | AXG93702.1 | OG0000440 | CP031097.1:3551573–3552427 + | 3-oxoacyl-ACP reductase [Clostridium botulinum] |
| adhEAGE29_11755 | AXG92419.1 | OG0001124 | CP031097.1:2070454–2073042 - | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPAGE29_08470 | AXG91812.1 | OG0001228 | CP031097.1:1400281–1401804 - | L-lactate permease [Clostridium botulinum] |
| rnfAAGE29_11615 | AXG92392.1 | OG0001328 | CP031097.1:2038812–2039390 - | electron transport complex protein RnfA [Clostridium botulinum] |
| crtAGE29_16290 | AXG93194.1 | OG0001639 | CP031097.1:2962891–2963673 + | crotonase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.