Assembly
ASM1406861v1
BioProject PRJNA592543 · BioSample SAMN13428652
GENOME-RESOLVED EVIDENCE
GCA_014068615.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3918289 bp · GC 28.000%
Completeness 99.490% · contamination 0.170%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCGNZ07_17940 | QMU00471.1 | OG0000061 | CP059677.1:3865217–3866563 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| ldhGNZ07_08185 | QMU02012.1 | OG0000131 | CP059677.1:1764788–1765744 - | L-lactate dehydrogenase [Clostridium botulinum] |
| accBGNZ07_17950 | QMU00473.1 | OG0000212 | CP059677.1:3867049–3867525 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdGNZ07_15620 | QMU00068.1 | OG0000229 | CP059677.1:3406158–3406988 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlGNZ07_15615 | QMU00067.1 | OG0000234 | CP059677.1:3404955–3406133 - | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| fabZGNZ07_17945 | QMU00472.1 | OG0000785 | CP059677.1:3866595–3867029 - | 3-hydroxyacyl-ACP dehydratase FabZ [Clostridium botulinum] |
| adhEGNZ07_01915 | QMU00846.1 | OG0001124 | CP059677.1:401306–403894 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPGNZ07_05370 | QMU01478.1 | OG0001228 | CP059677.1:1162564–1164087 + | L-lactate permease [Clostridium botulinum] |
| rnfAGNZ07_02050 | QMU00873.1 | OG0001328 | CP059677.1:434766–435344 + | electron transport complex protein RnfA [Clostridium botulinum] |
| crtGNZ07_15625 | QMU00069.1 | OG0001639 | CP059677.1:3407019–3407801 - | short-chain-enoyl-CoA hydratase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.