Assembly
ASM1679834v1
BioProject PRJNA693403 · BioSample SAMN17386352
GENOME-RESOLVED EVIDENCE
GCA_016798345.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3815652 bp · GC 28.000%
Completeness 99.490% · contamination 0.170%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCJMA41_17520 | QRC52788.1 | OG0000061 | CP068960.1:3768506–3769852 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| ldhJMA41_07435 | QRC54216.1 | OG0000131 | CP068960.1:1617000–1617956 - | L-lactate dehydrogenase [Clostridium botulinum] |
| accBJMA41_17530 | QRC52790.1 | OG0000212 | CP068960.1:3770338–3770814 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdJMA41_15340 | QRC52410.1 | OG0000229 | CP068960.1:3338310–3339140 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlJMA41_15335 | QRC52409.1 | OG0000234 | CP068960.1:3337107–3338285 - | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| fabZJMA41_17525 | QRC52789.1 | OG0000785 | CP068960.1:3769884–3770318 - | 3-hydroxyacyl-ACP dehydratase FabZ [Clostridium botulinum] |
| adhEJMA41_01820 | QRC53150.1 | OG0001124 | CP068960.1:385894–388482 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPJMA41_05025 | QRC53750.1 | OG0001228 | CP068960.1:1072246–1073769 + | L-lactate permease [Clostridium botulinum] |
| rnfAJMA41_01955 | QRC53177.1 | OG0001328 | CP068960.1:419070–419648 + | electron transport complex protein RnfA [Clostridium botulinum] |
| crtJMA41_15345 | QRC52411.1 | OG0001639 | CP068960.1:3339171–3339953 - | short-chain-enoyl-CoA hydratase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.