Assembly
ASM1683866v1
BioProject PRJNA698972 · BioSample SAMN17763437
GENOME-RESOLVED EVIDENCE
GCA_016838665.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4090796 bp · GC 28.000%
Completeness 98.980% · contamination 0.760%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCJQS73_19450 | QRI53528.1 | OG0000061 | CP069280.1:4042352–4043698 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| ldhJQS73_08420 | QRI55106.1 | OG0000131 | CP069280.1:1749691–1750647 - | L-lactate dehydrogenase [Clostridium botulinum] |
| accBJQS73_19460 | QRI53530.1 | OG0000212 | CP069280.1:4044184–4044660 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdJQS73_17240 | QRI53144.1 | OG0000229 | CP069280.1:3602504–3603334 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlJQS73_17235 | QRI53143.1 | OG0000234 | CP069280.1:3601301–3602479 - | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| fabZJQS73_19455 | QRI53529.1 | OG0000785 | CP069280.1:4043730–4044164 - | 3-hydroxyacyl-ACP dehydratase FabZ [Clostridium botulinum] |
| adhEJQS73_01845 | QRI53892.1 | OG0001124 | CP069280.1:391165–393753 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPJQS73_05625 | QRI54584.1 | OG0001228 | CP069280.1:1158629–1160152 + | L-lactate permease [Clostridium botulinum] |
| rnfAJQS73_01980 | QRI53919.1 | OG0001328 | CP069280.1:424663–425241 + | electron transport complex protein RnfA [Clostridium botulinum] |
| crtJQS73_17245 | QRI53145.1 | OG0001639 | CP069280.1:3603354–3604136 - | short-chain-enoyl-CoA hydratase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.