Assembly
ASM2283314v1
BioProject PRJNA610151 · BioSample SAMN14278160
GENOME-RESOLVED EVIDENCE
GCA_022833145.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3938593 bp · GC 28.000%
Completeness 99.490% · contamination 0.170%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 4/8 | 0.500 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabG | 3-oxoacyl-ACP reductase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 2 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCG8E05_18195 | UOJ22820.1 | OG0000061 | CP050251.1:3839757–3841103 + | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZG8E05_07015 | UOJ20781.1 | OG0000119 | CP050251.1:1439489–1441180 - | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhG8E05_10090 | UOJ21338.1 | OG0000131 | CP050251.1:2122904–2123860 + | L-lactate dehydrogenase [Clostridium botulinum] |
| accBG8E05_18185 | UOJ22818.1 | OG0000212 | CP050251.1:3838796–3839272 + | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdG8E05_01805 | UOJ19818.1 | OG0000229 | CP050251.1:359002–359832 + | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlG8E05_01810 | UOJ19819.1 | OG0000234 | CP050251.1:359857–361035 + | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| fabGG8E05_04425 | UOJ20306.1 | OG0000440 | CP050251.1:940781–941635 + | 3-oxoacyl-ACP reductase [Clostridium botulinum] |
| fabZG8E05_18190 | UOJ22819.1 | OG0000785 | CP050251.1:3839291–3839725 + | 3-hydroxyacyl-ACP dehydratase FabZ [Clostridium botulinum] |
| adhEG8E05_15980 | UOJ22442.1 | OG0001124 | CP050251.1:3395417–3398005 - | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPG8E05_12815 | UOJ21863.1 | OG0001228 | CP050251.1:2708416–2709939 - | L-lactate permease [Clostridium botulinum] |
| rnfAG8E05_15845 | UOJ22415.1 | OG0001328 | CP050251.1:3363790–3364368 - | electron transport complex protein RnfA [Clostridium botulinum] |
| crtG8E05_01800 | UOJ19817.1 | OG0001639 | CP050251.1:358200–358982 + | short-chain-enoyl-CoA hydratase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.