Assembly
ASM2794366v1
BioProject PRJDB12098 · BioSample SAMD00396923
GENOME-RESOLVED EVIDENCE
GCA_027943665.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4138332 bp · GC 28.000%
Completeness 99.490% · contamination 0.170%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 1/3 | 0.333 | Partial/absent |
| ethanol_entry_support | 0/1 | 0.000 | Partial/absent |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 4/4 | 1.000 | Complete marker set |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| bcd | butyryl-CoA dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| bcdCBOS2020_31080 | BDB03034.1 | OG0000034 | AP025140.1:3339892–3341031 - | butyryl-CoA dehydrogenase [Clostridium botulinum] |
| accCCBOS2020_35190 | BDB03445.1 | OG0000061 | AP025140.1:3797775–3799121 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| ldhCBOS2020_14960 | BDB01422.1 | OG0000131 | AP025140.1:1622981–1623937 - | L-lactate dehydrogenase [Clostridium botulinum] |
| accBCBOS2020_35210 | BDB03447.1 | OG0000212 | AP025140.1:3799606–3800082 - | acetyl-CoA carboxylase biotin carboxyl carrier protein subunit [Clostridium botulinum] |
| hbdCBOS2020_31100 | BDB03036.1 | OG0000229 | AP025140.1:3342428–3343258 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlCBOS2020_31090 | BDB03035.1 | OG0000234 | AP025140.1:3341225–3342403 - | acetyl-CoA acetyltransferase [Clostridium botulinum] |
| fabZCBOS2020_31540 | BDB03080.1 | OG0001208 | AP025140.1:3386031–3387290 - | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| glcACBOS2020_09990 | BDB00925.1 | OG0001228 | AP025140.1:1079067–1080590 + | L-lactate permease [Clostridium botulinum] |
| crtCBOS2020_31110 | BDB03037.1 | OG0001639 | AP025140.1:3343278–3344060 - | crotonase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.