Assembly
ASM2841669v1
BioProject PRJNA806856 · BioSample SAMN25937290
GENOME-RESOLVED EVIDENCE
GCA_028416695.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4170326 bp · GC 28.000%
Completeness 98.980% · contamination —%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCMHI65_003739 | WCJ80968.1 | OG0000061 | CP102917.1:3930137–3931483 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| ldhMHI65_001602 | WCJ82514.1 | OG0000131 | CP102917.1:1688131–1689087 - | L-lactate dehydrogenase [Clostridium botulinum] |
| accBMHI65_003741 | WCJ80970.1 | OG0000212 | CP102917.1:3931969–3932445 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdMHI65_003293 | WCJ80579.1 | OG0000229 | CP102917.1:3488034–3488864 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlMHI65_003292 | WCJ80578.1 | OG0000234 | CP102917.1:3486831–3488009 - | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| fabZMHI65_003740 | WCJ80969.1 | OG0000785 | CP102917.1:3931515–3931949 - | 3-hydroxyacyl-ACP dehydratase FabZ [Clostridium botulinum] |
| adhEMHI65_000375 | WCJ81344.1 | OG0001124 | CP102917.1:387467–390055 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPMHI65_001061 | WCJ81996.1 | OG0001228 | CP102917.1:1102827–1104350 + | L-lactate permease [Clostridium botulinum] |
| rnfAMHI65_000402 | WCJ81371.1 | OG0001328 | CP102917.1:421110–421688 + | electron transport complex protein RnfA [Clostridium botulinum] |
| crtMHI65_003294 | WCJ80580.1 | OG0001639 | CP102917.1:3488884–3489666 - | short-chain-enoyl-CoA hydratase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.