Assembly
ASM2996236v1
BioProject PRJNA610151 · BioSample SAMN14278157
GENOME-RESOLVED EVIDENCE
GCA_029962365.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 3989081 bp · GC 28.000%
Completeness 99.240% · contamination 0.170%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 4/8 | 0.500 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabG | 3-oxoacyl-ACP reductase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 2 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCHEQ52_00365 | WGZ44826.1 | OG0000061 | CP050820.1:59845–61191 + | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| fabZHEQ52_07830 | WGZ46171.1 | OG0000119 | CP050820.1:1584501–1586192 - | 3-hydroxyacyl-ACP dehydratase [Clostridium botulinum] |
| ldhHEQ52_10945 | WGZ46724.1 | OG0000131 | CP050820.1:2267915–2268871 + | L-lactate dehydrogenase [Clostridium botulinum] |
| accBHEQ52_00355 | WGZ44824.1 | OG0000212 | CP050820.1:58884–59360 + | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdHEQ52_02600 | WGZ45210.1 | OG0000229 | CP050820.1:504012–504842 + | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlHEQ52_02605 | WGZ45211.1 | OG0000234 | CP050820.1:504867–506045 + | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| fabGHEQ52_05235 | WGZ45696.1 | OG0000440 | CP050820.1:1085794–1086648 + | 3-oxoacyl-ACP reductase [Clostridium botulinum] |
| fabZHEQ52_00360 | WGZ44825.1 | OG0000785 | CP050820.1:59379–59813 + | 3-hydroxyacyl-ACP dehydratase FabZ [Clostridium botulinum] |
| adhEHEQ52_16850 | WGZ47831.1 | OG0001124 | CP050820.1:3540433–3543021 - | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPHEQ52_13670 | WGZ47244.1 | OG0001228 | CP050820.1:2853432–2854955 - | L-lactate permease [Clostridium botulinum] |
| rnfAHEQ52_16715 | WGZ47804.1 | OG0001328 | CP050820.1:3508809–3509387 - | electron transport complex protein RnfA [Clostridium botulinum] |
| crtHEQ52_02595 | WGZ45209.1 | OG0001639 | CP050820.1:503210–503992 + | short-chain-enoyl-CoA hydratase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.