Assembly
ASM3122868v1
BioProject PRJNA286240 · BioSample SAMN30265469
GENOME-RESOLVED EVIDENCE
GCA_031228685.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4330354 bp · GC 28.000%
Completeness 99.490% · contamination 0.680%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCQA656_18360 | WMU97666.1 | OG0000061 | CP121696.1:4021014–4022360 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| ldhQA656_08090 | WMU99225.1 | OG0000131 | CP121696.1:1778441–1779397 - | L-lactate dehydrogenase [Clostridium botulinum] |
| accBQA656_18370 | WMU97668.1 | OG0000212 | CP121696.1:4022846–4023322 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdQA656_16040 | WMU97259.1 | OG0000229 | CP121696.1:3555248–3556078 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlQA656_16035 | WMU97258.1 | OG0000234 | CP121696.1:3554045–3555223 - | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| fabZQA656_18365 | WMU97667.1 | OG0000785 | CP121696.1:4022392–4022826 - | 3-hydroxyacyl-ACP dehydratase FabZ [Clostridium botulinum] |
| adhEQA656_01895 | WMU98042.1 | OG0001124 | CP121696.1:406337–408925 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPQA656_05395 | WMU98706.1 | OG0001228 | CP121696.1:1173140–1174663 + | L-lactate permease [Clostridium botulinum] |
| crtQA656_16045 | WMU97260.1 | OG0001639 | CP121696.1:3556098–3556880 - | short-chain-enoyl-CoA hydratase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.