Assembly
ASM3356153v1
BioProject PRJNA999584 · BioSample SAMN36736628
GENOME-RESOLVED EVIDENCE
GCA_033561535.1
Clostridium sp. JS66 · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 6312792 bp · GC 30.000%
Completeness 99.380% · contamination 3.110%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 4/8 | 0.500 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 0/6 | 0.000 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 3 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 2 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabG | 3-oxoacyl-ACP reductase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| lctP | L-lactate permease | 2 |
| ldh | L-lactate dehydrogenase | 2 |
| crt | crotonase/enoyl-CoA hydratase | 4 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 3 |
| thl | acetyl-CoA acetyltransferase/thiolase | 3 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCQ6H37_29165 | WPC41873.1 | OG0000061 | CP131467.1:6266710–6268056 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium sp. JS66] |
| ldhQ6H37_16335 | WPC39480.1 | OG0000131 | CP131467.1:3538150–3539106 + | L-lactate dehydrogenase [Clostridium sp. JS66] |
| ldhQ6H37_23730 | WPC40875.1 | OG0000131 | CP131467.1:5132776–5133765 - | L-lactate dehydrogenase [Clostridium sp. JS66] |
| accBQ6H37_06995 | WPC43212.1 | OG0000212 | CP131467.1:1460182–1460394 + | acetyl-CoA carboxylase biotin carboxyl carrier protein subunit [Clostridium sp. JS66] |
| accBQ6H37_29175 | WPC41875.1 | OG0000212 | CP131467.1:6268540–6269058 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium sp. JS66] |
| hbdQ6H37_02020 | WPC42278.1 | OG0000229 | CP131467.1:431431–432276 + | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium sp. JS66] |
| hbdQ6H37_02525 | WPC42358.1 | OG0000229 | CP131467.1:549548–550393 + | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium sp. JS66] |
| hbdQ6H37_22045 | WPC40559.1 | OG0000229 | CP131467.1:4789161–4790006 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium sp. JS66] |
| thlQ6H37_02025 | WPC42279.1 | OG0000234 | CP131467.1:432346–433524 + | acetyl-CoA C-acetyltransferase [Clostridium sp. JS66] |
| thlQ6H37_02530 | WPC42359.1 | OG0000234 | CP131467.1:550416–551594 + | acetyl-CoA C-acetyltransferase [Clostridium sp. JS66] |
| thlQ6H37_22040 | WPC40558.1 | OG0000234 | CP131467.1:4787916–4789094 - | acetyl-CoA C-acetyltransferase [Clostridium sp. JS66] |
| crtQ6H37_05975 | WPC43020.1 | OG0000330 | CP131467.1:1253944–1254849 + | bifunctional enoyl-CoA hydratase/phosphate acetyltransferase [Clostridium sp. JS66] |
| fabZQ6H37_29170 | WPC41874.1 | OG0000785 | CP131467.1:6268049–6268510 - | 3-hydroxyacyl-ACP dehydratase FabZ [Clostridium sp. JS66] |
| adhEQ6H37_07690 | WPC43343.1 | OG0001124 | CP131467.1:1618816–1621437 - | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium sp. JS66] |
| adhEQ6H37_15265 | WPC39275.1 | OG0001124 | CP131467.1:3268314–3270890 - | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium sp. JS66] |
| adhEQ6H37_15270 | WPC39276.1 | OG0001124 | CP131467.1:3271081–3273714 - | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium sp. JS66] |
| lctPQ6H37_19185 | WPC40013.1 | OG0001228 | CP131467.1:4103760–4105346 - | L-lactate permease [Clostridium sp. JS66] |
| lctPQ6H37_21125 | WPC40385.1 | OG0001228 | CP131467.1:4553023–4554531 - | L-lactate permease [Clostridium sp. JS66] |
| crtQ6H37_02015 | WPC42277.1 | OG0001639 | CP131467.1:430589–431368 + | short-chain-enoyl-CoA hydratase [Clostridium sp. JS66] |
| crtQ6H37_02520 | WPC44708.1 | OG0001639 | CP131467.1:548725–549504 + | short-chain-enoyl-CoA hydratase [Clostridium sp. JS66] |
| crtQ6H37_22050 | WPC40560.1 | OG0001639 | CP131467.1:4790087–4790866 - | short-chain-enoyl-CoA hydratase [Clostridium sp. JS66] |
| fabGQ6H37_26135 | WPC41338.1 | OG0004652 | CP131467.1:5632558–5633316 - | 3-oxoacyl-ACP reductase family protein [Clostridium sp. JS66] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.