Assembly
ASM5682433v1
BioProject PRJNA759433 · BioSample SAMN21169057
GENOME-RESOLVED EVIDENCE
GCA_056824335.1
Clostridium botulinum · Complete Genome
View versioned NCBI assembly →
Quality metadata
Genome size 4086676 bp · GC 28.000%
Completeness 99.490% · contamination 0.170%
Pathway marker completeness
| Module | Markers | Fraction | Call |
|---|---|---|---|
| bcd_etf_module | 0/3 | 0.000 | Partial/absent |
| ethanol_entry_support | 1/1 | 1.000 | Complete marker set |
| fatty_acid_biosynthesis_core | 3/8 | 0.375 | Partial/absent |
| lactate_entry_support | 2/2 | 1.000 | Complete marker set |
| reverse_beta_oxidation_core | 3/4 | 0.750 | Partial/absent |
| rnf_complex | 1/6 | 0.167 | Partial/absent |
Detected markers
| Marker | Function | Copies |
|---|---|---|
| rnfA | Rnf complex subunit A | 1 |
| adhE | bifunctional acetaldehyde-CoA/alcohol dehydrogenase | 1 |
| accB | acetyl-CoA carboxylase biotin carboxyl carrier protein | 1 |
| accC | acetyl-CoA carboxylase biotin carboxylase | 1 |
| fabZ | 3-hydroxyacyl-ACP dehydratase | 1 |
| lctP | L-lactate permease | 1 |
| ldh | L-lactate dehydrogenase | 1 |
| crt | crotonase/enoyl-CoA hydratase | 1 |
| hbd | 3-hydroxybutyryl-CoA dehydrogenase | 1 |
| thl | acetyl-CoA acetyltransferase/thiolase | 1 |
Profile-HMM marker predictions
Approved candidates and review records are genomic predictions, not evidence of expression or MCFA production. Excluded matches remain visible for audit.
| Marker / protein | Confidence | Review status | Score / coverage | Supporting evidence |
|---|---|---|---|---|
| No profile-HMM predictions for this assembly. | ||||
Protein-level evidence
| Gene | Protein | Orthogroup | Coordinates | Annotation |
|---|---|---|---|---|
| accCK8O63_003750 | YEB05797.1 | OG0000061 | CP102849.1:4039530–4040876 - | acetyl-CoA carboxylase biotin carboxylase subunit [Clostridium botulinum] |
| ldhK8O63_001598 | YEB07324.1 | OG0000131 | CP102849.1:1768267–1769223 - | L-lactate dehydrogenase [Clostridium botulinum] |
| accBK8O63_003752 | YEB05799.1 | OG0000212 | CP102849.1:4041362–4041838 - | acetyl-CoA carboxylase biotin carboxyl carrier protein [Clostridium botulinum] |
| hbdK8O63_003287 | YEB05389.1 | OG0000229 | CP102849.1:3580398–3581228 - | 3-hydroxybutyryl-CoA dehydrogenase [Clostridium botulinum] |
| thlK8O63_003286 | YEB05388.1 | OG0000234 | CP102849.1:3579195–3580373 - | acetyl-CoA C-acetyltransferase [Clostridium botulinum] |
| fabZK8O63_003751 | YEB05798.1 | OG0000785 | CP102849.1:4040908–4041342 - | 3-hydroxyacyl-ACP dehydratase FabZ [Clostridium botulinum] |
| adhEK8O63_000393 | YEB06185.1 | OG0001124 | CP102849.1:432834–435422 + | bifunctional acetaldehyde-CoA/alcohol dehydrogenase [Clostridium botulinum] |
| lctPK8O63_001097 | YEB06838.1 | OG0001228 | CP102849.1:1207967–1209490 + | L-lactate permease [Clostridium botulinum] |
| rnfAK8O63_000420 | YEB06211.1 | OG0001328 | CP102849.1:466294–466872 + | electron transport complex protein RnfA [Clostridium botulinum] |
| crtK8O63_003288 | YEB05390.1 | OG0001639 | CP102849.1:3581259–3582041 - | short-chain-enoyl-CoA hydratase [Clostridium botulinum] |
These are genomic annotation predictions. Experimental activity, expression and metabolite production require separate evidence.