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SPECIES · STRAIN AND FUNCTION PROFILE

Clostridium acetobutylicum

Reported/putative producer

Imported from the NCBI assembly data report; MCFA production is not implied.

1strain profiles
4assemblies
39MCFA marker genes
50%strain metadata coverage

Name, taxonomy and strain identifiers

Curation status: Exact match imported · Culture collection identifier · checked 2026-07-29. Exact strain-level match; curated BacDive fields imported.

Taxonomic record

Scientific name
Clostridium acetobutylicum
Rank
species
NCBI Taxonomy
Not resolved at this level
Database role
Reported/putative producer

2291, W Type strain

Full name
Clostridium acetobutylicum McCoy et al. 1926 emend. Keis et al. 2001
Culture collections
DSM 792, ATCC 824, VKM B-1787, CCUG 42182 C, LMG 5710, CECT 508, IFO 13948, JCM 1419, BCRC 10639, CCUG 42182, IAM 19013, KCTC 1790, NBRC 13948, NCIMB 8052, NRRL B-527
History
Not curated
BacDive
Strain 2529

Morphology and observable traits

StrainGram stainCell shapeMotilitySpore formationColony morphology
2291, WNot curatedNot curatedNot curatedNot curatedNot curated

Culture and growth conditions

StrainMediumTemperaturepHAtmosphere/result
2291, WCLOSTRIDIUM ACETOBUTYLICUM MEDIUM (DSMZ Medium 411)DSMZ Medium 411 · Name: CLOSTRIDIUM ACETOBUTYLICUM MEDIUM (DSMZ Medium 411) Composition: Potato 200.0 g/l D-Glucose 6.0 g/l CaCO3 2.0 g/l L-Cysteine HCl x H2O 0.5 g/l Sodium resazurin 0.0005 g/l Distilled water37.00 °C—— · yes
2291, WPY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)DSMZ Medium 104 · Name: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l Na2CO3 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water37.00 °C—— · yes

Documented condition ranges

ProfilepHTemperatureElectron donorsSubstrates/reactor
BacDive documented cultivation conditions?–?; optimum ?37.00–37.00 °C; optimum ?Not curated—; culture-collection cultivation
Species-level documented cultivation conditions?–?; optimum ?35.00–37.00 °C; optimum ?Not curated—; species-level culture records

Physiology and metabolism

Documented substrates and products

Only approved assertions are shown. Results may be strain- and condition-specific.

RelationshipCompound/source termEvidenceConfidenceCondition
No approved strain-level phenotype assertions yet.

Predicted metabolic capabilities

Genome-derived hypotheses requiring review—not demonstrated substrates or products.

CapabilityBasis/statusConfidenceAssembliesLimitation
reverse beta-oxidation potentialGenomic prediction · Needs review0.600GCA_000218855.1, GCA_000191905.1Complete marker-rule set; not evidence of expression, flux, or product formation.
CoA-transfer terminal-step potentialGenomic prediction · Needs review0.500GCA_000191905.1, GCA_000218855.1, GCA_000008765.1A terminal CoA-transfer annotation is supportive but not product-specific.
lactate metabolism potentialGenomic prediction · Needs review0.400GCA_000191905.1, GCA_000218855.1, GCA_000008765.1LDH presence does not establish reaction direction or growth on lactate.
ethanol metabolism potentialGenomic prediction · Needs review0.400GCA_000218855.1AdhE can participate in ethanol production or consumption depending on physiology.

Isolation, environment and safety

StrainIsolation sourceCategory/locationOxygen toleranceBiosafetyGC content
2291, Wcornmeal—Not curated1Risk group (German classification)—%

Sequence information

Genome assemblies

AccessionSourceLevelSize/GCQualityBioProject
GCF_000218855.1NCBI RefSeq—— bp / —%—% complete; —% contaminationPRJNA63241
GCA_000008765.1NCBI GenBankComplete Genome4132880 bp / 31.000%97.920% complete; 0.930% contaminationPRJNA77
GCA_000191905.1NCBI GenBankComplete Genome4132226 bp / 31.000%97.840% complete; 0.930% contaminationPRJNA50455
GCA_000218855.1NCBI GenBankComplete Genome4145581 bp / 31.000%98.050% complete; 0.830% contaminationPRJNA63241

Marker and ribosomal sequences

StrainTypeAccessionLengthDescription
2291, W16S rRNAU161661411 bpClostridium acetobutylicum ATCC 824 16S ribosomal RNA (rrn) gene, partial sequence
2291, W16S rRNAU17030554 bpClostridium acetobutylicum DSM 792 16S ribosomal RNA (rrn) gene, partial sequence
2291, W16S rRNAX780701389 bpC.acetobutylicum (ATCC 824, T) 16S rRNA
2291, W16S rRNAAB5951311467 bpClostridium acetobutylicum gene for 16S ribosomal RNA, partial sequence, strain: JCM 1419
2291, W16S rRNAS467351411 bp16S rRNA [Clostridium acetobutylicum, NCIMB 8052, rRNA, 1411 nt]
2291, W16S rRNAU161651416 bpClostridium acetobutylicum NCIMB8052 16S ribosomal RNA (rrn) gene, partial sequence.
2291, WGenomeGCA_000008765— bpASM876v1 assembly for Clostridium acetobutylicum ATCC 824
2291, WGenomeGCA_000016965— bpASM1696v1 assembly for Clostridium beijerinckii NCIMB 8052

MCFA pathways and genes

Reverse β-oxidation (chain elongation)5 of 5 functions100%100%
Fatty acid biosynthesis (FAS II)3 of 7 functions43%43%
Show 39 annotation-derived MCFA genes
GeneLocusFunctionProteinOrthogroup
ctfaCA_P0163CoA transferase / butyrate-acetoacetate CoA-transferase (2.8.3.9)AAK76908.1OG0000158
ctfbCA_P0164CoA transferase / butyrate-acetoacetate CoA-transferase (2.8.3.9)AAK76909.1OG0000158
ldhCA_C0267L-lactate dehydrogenase (1.1.1.27)AAK78248.1OG0000131
fadBCA_C20123-hydroxybutyryl-CoA dehydratase (4.2.1.55)AAK79971.1OG0014900
fadBCA_C20163-hydroxybutyryl-CoA dehydratase (4.2.1.55)AAK79975.1OG0014902
bcdCA_C2711Butyryl-CoA dehydrogenase (1.3.8.1)AAK80657.1OG0001311
crtCA_C27123-hydroxybutyryl-CoA dehydratase (4.2.1.55)AAK80658.1OG0001394
thlCA_C2873Acetyl-CoA acetyltransferase (2.3.1.9)AAK80816.1OG0000234
fabGCA_C34623-oxoacyl-ACP reductase (1.1.1.100)AAK81391.1OG0004652
accACA_C3568Acetyl-CoA carboxylase (6.4.1.2)AAK81491.1OG0000885
accDCA_C3569Acetyl-CoA carboxylase (6.4.1.2)AAK81492.1OG0000403
ldhCEA_G0273L-lactate dehydrogenase (1.1.1.27)ADZ19314.1OG0000131
fadBCEA_G05713-hydroxybutyryl-CoA dehydratase (4.2.1.55)ADZ19610.1OG0018950
crtCEA_G20273-hydroxybutyryl-CoA dehydratase (4.2.1.55)ADZ21064.1OG0014900
crtCEA_G20313-hydroxybutyryl-CoA dehydratase (4.2.1.55)ADZ21068.1OG0014902
hbdCEA_G27163-hydroxybutyryl-CoA dehydrogenase (1.1.1.157)ADZ21753.1OG0000229
bcdCEA_G2719Butyryl-CoA dehydrogenase (1.3.8.1)ADZ21756.1OG0001311
thlCEA_G2880Acetyl-CoA acetyltransferase (2.3.1.9)ADZ21917.1OG0000234
fabGCEA_G34663-oxoacyl-ACP reductase (1.1.1.100)ADZ22503.1OG0004652
accACEA_G3575Acetyl-CoA carboxylase (6.4.1.2)ADZ22612.1OG0000885
accDCEA_G3576Acetyl-CoA carboxylase (6.4.1.2)ADZ22613.1OG0000403
thilCEA_P0077Acetyl-CoA acetyltransferase (2.3.1.9)ADZ22860.1OG0000234
ctfaCEA_P0161CoA transferase / butyrate-acetoacetate CoA-transferase (2.8.3.9)ADZ22944.1OG0000158
ctfbCEA_P0162CoA transferase / butyrate-acetoacetate CoA-transferase (2.8.3.9)ADZ22945.1OG0000158
thlSMB_G2909Acetyl-CoA acetyltransferase (2.3.1.9)AEI32591.1OG0000234
hbdSMB_G27433-hydroxybutyryl-CoA dehydrogenase (1.1.1.157)AEI33291.1OG0000229
fadJSMB_G20483-hydroxybutyryl-CoA dehydratase (4.2.1.55)AEI33740.1OG0014902
bcdSMB_G2746Butyryl-CoA dehydrogenase (1.3.8.1)AEI33941.1OG0001311
accDSMB_G3610Acetyl-CoA carboxylase (6.4.1.2)AEI34012.1OG0000403
ldh1SMB_G0272L-lactate dehydrogenase (1.1.1.27)AEI34058.1OG0000131
fabGSMB_G35003-oxoacyl-ACP reductase (1.1.1.100)AEI34322.1OG0004652
fadBSMB_G05703-hydroxybutyryl-CoA dehydratase (4.2.1.55)AEI34354.1OG0018950
fadBSMB_G20443-hydroxybutyryl-CoA dehydratase (4.2.1.55)AEI34355.1OG0014900
fabHSMB_G20433-oxoacyl-ACP synthase III (2.3.1.180)AEI34559.1OG0000222
adheSMB_P034Bifunctional acetaldehyde-CoA/alcohol dehydrogenase (1.2.1.10)AEI34777.1OG0012903
thilSMB_P077Acetyl-CoA acetyltransferase (2.3.1.9)AEI34820.1OG0000234
adhe1SMB_P160Bifunctional acetaldehyde-CoA/alcohol dehydrogenase (1.2.1.10)AEI34903.1OG0001124
ctfaSMB_P161CoA transferase / butyrate-acetoacetate CoA-transferase (2.8.3.9)AEI34904.1OG0000158
ctfbSMB_P162CoA transferase / butyrate-acetoacetate CoA-transferase (2.8.3.9)AEI34905.1OG0000158

Curator-approved marker pathway assessments

These summaries use approved candidate predictions only and update after every review decision.

AssemblyPathway ruleApproved markersStatus
No approved-marker pathway assessments.

Profile-HMM marker predictions

Approved candidates and unresolved review calls are genomic hypotheses, not evidence of expression or MCFA production.

MarkerAssembly / proteinConfidence and statusSupportReview history
No active marker predictions for this organism.

Literature and provenance

StudyDOIPublic data
No organism-linked literature indexed yet.

Profile evidence

External databases can change. mcfaKB records source links and separates experimentally reported, curator-entered and predicted information.